Evaluation of the efficacy of combination of glutaraldehyde and quaternary ammonium compound disinfectant against different isolated C. perfringens strains recovered from broilers

Document Type : Original researches

Abstract

   
Necrotic enteritis caused by Clostridium perfringens has been linked to severe economic losses in the poultry industry and represents a serious threat to public health due to spore-forming ability and its role as foodborne pathogen. Although chemical disinfection plays a role in preventing and controlling the spread of bacterial diseases in livestock and poultry housing, little is known about its efficiency against spore-forming C. perfringens. This study was designed to investigate the sporicidal activity of a combination of quaternary ammonium and glutaraldehyde disinfectant (TH4) against previously isolated ten C. perfringens strains according to European Standard EN 1276, 2019 application method at different concentrations (0.25%, 0.5%, 1%) with different contact times (0, 1, 5, 15, 30, 60 minutes). C. perfringens strains were identified, toxinotyped, and screened for the presence of QAC resistance genes (qacED1 and qacA/B). Five C. perfringens isolates were verified to produce toxinotype A, while the remaining five isolates were confirmed to produce toxinotype G. Screening for qacED1 and qacA/B resistance genes revealed their presence in six and four isolates, respectively. TH4 0.25% and 0.5% tested in this study didn’t give the required five log10 CFU reduction in the spore count in five C. perfringens at all exposure times, while concentration of 1% achieved the required five log reduction in the count of C. perfringens spores for relatively long contact times of 60 minutes in five C. perfringens type A isolates. These findings recommend the use of concentration of TH4 1% and increase exposure time to 60 minutes to obtain effective sporicidal activity against C. perfringens strains.

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References

Abdallah M, Benoliel C, Drider D, Dhulster P., Chihib NE. 2014. Biofilm formation and persistence on abiotic surfaces in the context of food and medical environments. Archives of microbiology 196(7): 453-472.
Acsa I, Lilly Caroline B, Philip Njeru N, Lucy Wanjiru N. 2021. Preliminary study on disinfectant susceptibility/resistance profiles of bacteria isolated from slaughtered village free-range chickens in Nairobi, Kenya. International Journal of Microbiology 2021 1-7.
Almatrafi R, Banawas S, Sarker MR. 2023. Divalent Cation Signaling in Clostridium perfringens Spore Germination. Microorganisms 11(3): 591.
Brantner CA, Hannah RM. Burans JP, Pope RK. 2014. Inactivation and ultrastructure analysis of Bacillus spp. and Clostridium perfringens spores. Microscopy Microanalysis 20(1): 238-244.
Chapman JS. 2003. Disinfectant resistance mechanisms, cross-resistance, and co-resistance. International biodeterioration biodegradation 51(4): 271-276.
Chidambaranathan AS, Balasubramanium M. 2019. Comprehensive review and comparison of the disinfection techniques currently available in the literature. Journal of Prosthodontics 28(2): e849-e856.
Chuanchuen R, Khemtong S, Padungtod P, 2007. Occurrence of qacE/qacED1 genes and their correlation with class 1 integrons in Salmonella enterica isolates from poultry and swine. Southeast Asian J Trop Med Public Health 38(5): 855-862.
Cooper KK, Songer JG. 2010. Virulence of Clostridium perfringens in an experimental model of poultry necrotic enteritis. Veterinary microbiology 142(3-4): 323-328.
Dar PS, Wani SA, Wani AH, Hussain I, Maqbool R, Ganaie MY, Kashoo ZA, Qureshi S. 2017. Isolation, identification and molecular characterization of Clostridium perfringens from poultry in Kashmir valley, India. Journal of Entomology Zoology Studies 5(5): 409-414.
Davies R, Wales A. 2019. Antimicrobial resistance on farms: a review including biosecurity and the potential role of disinfectants in resistance selection. Comprehensive reviews in food science and food safety18(3): 753-774.
 Fathima S, Hakeem WGA, Shanmugasundaram R, Selvaraj RK. 2022. Necrotic enteritis in broiler chickens: A review on the pathogen, pathogenesis, and prevention. Microorganisms 10(10): 1958.
Gharaibeh S, Al Rifai R, Al-Majali A. 2010. Molecular typing and antimicrobial susceptibility of Clostridium perfringens from broiler chickens. Anaerobe 16(6): 586-589.
He W, Goes EC, Wakaruk J, Barreda DR, Korver DR. 2022. A poultry subclinical necrotic enteritis disease model based on natural Clostridium perfringens uptake. Frontiers in Physiology 13 788592.
Hosny RA, Gaber AF, Sorour HK. 2021. Bacteriophage mediated control of necrotic enteritis caused by C. perfringens in broiler chickens. Veterinary Research Communications (45): 409-421.
Ibrahim WA, Marouf SA, Erfan AM, Nasef SA, El Jakee JK. 2019. The occurrence of disinfectant and antibiotic-resistant genes in Escherichia coli isolated from chickens in Egypt. Veterinary world 12(1): 141.
Jaglic Z, Cervinkova D. 2012. Genetic basis of resistance to quaternary ammonium compounds--the qac genes and their role: a review. Veterinarni Medicina 57(6).
Jang Y, Lee K, Yun S, Lee M, Song J, Chang B, Choe Nh. 2017. Efficacy evaluation of commercial disinfectants by using Salmonella enterica serovar Typhimurium as a test organism. Journal of veterinary science18(2): 209-216.
 Jennings MC, Minbiole KP, Wuest WM. 2015. Quaternary ammonium compounds: an antimicrobial mainstay and platform for innovation to address bacterial resistance. ACS infectious diseases 1(7): 288-303.
Jiang L, Li M, Tang J, Zhao X, Zhang J, Zhu H, Yu X, Li Y, Feng T, Zhang X. 2018. Effect of different disinfectants on bacterial aerosol diversity in poultry houses. Frontiers in microbiology 9 2113.
Kamal MA, Khalaf MA, Ahmed ZAM, El Jakee J. 2019. Evaluation of the efficacy of commonly used disinfectants against isolated chlorine-resistant strains from drinking water used in Egyptian cattle farms. Veterinary world 12(12): 2025.
Keyburn AL, Yan XX, Bannam T., Van Immerseel F, Rood JI, Moore RJ. 2010. Association between avian necrotic enteritis and Clostridium perfringens strains expressing NetB toxin. Veterinary research 41(2).
Kücken D, Feucht HH, Kaulfers PM. 2000. Association of qacE and qacE Δ1 with multiple resistance to antibiotics and antiseptics in clinical isolates of Gram-negative bacteria. FEMS Microbiology Letters 183(1): 95-98.
Li Y, Song Y, Huang Z, Mei L, Jiang M, Wang D, Wei Q. 2023. Screening of Staphylococcus aureus for disinfection evaluation and transcriptome analysis of high tolerance to chlorine-containing disinfectants. Microorganisms 11(2): 475.
Malmarugan S, Boobalan A, Dorairajan N. 2012. Necrotic Enteritis in broiler and layer farms in Tamil Nadu, India. International Journal for Agro Veterinary Medical Sciences 6(4): 241-249.
McSharry S, Koolman L, Whyte P, Bolton D. 2021. Investigation of the effectiveness of disinfectants used in meat-processing facilities to control Clostridium sporogenes and Clostridioides difficile spores. Foods 10(6): 1436.
Merati R, Temim S, AAAF, M. 2017. Identification and characterization of Clostridium perfringens isolated from necrotic enteritis in broiler chickens in Tiaret, Western Algeria. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 23(4).
Mohiuddin M, Song Z, Liao S, Qi N, Li J, Lv M, Lin X, Cai H, Hu J, Liu S. 2023. Animal Model Studies, Antibiotic Resistance and Toxin Gene Profile of NE Reproducing Clostridium perfringens Type A and Type G Strains Isolated from Commercial Poultry Farms in China. Microorganisms 11(3): 622.
Mora ZV, dl, Macías-Rodríguez ME, Arratia-Quijada J, Gonzalez-Torres YS, Nuño K, Villarruel-López A. 2020. Clostridium perfringens as foodborne pathogen in broiler production: pathophysiology and potential strategies for controlling necrotic enteritis. Animals 10(9): 1718.
Møretrø T, Vestby L, Nesse L, Storheim S, Kotlarz K, Langsrud S. 2009. Evaluation of efficacy of disinfectants against Salmonella from the feed industry. Journal of Applied Microbiology 106(3): 1005-1012.
Nasr SA, Ali MM, Hamoda A, Hatem E, El Agrab HM, Moubarak ST, Samaha HA, Zahran O. 2014. Sporicidal Activity of some Disinfectants Against Clostridium Perfringens Isolated from Broiler Poultry Litter. Alexandria Journal for Veterinary Sciences 42(1).
Ni X, Song Z, Zeng D, Zheng X, Gong JhJ,.2009. Isolation, identification and genotyping of Clostridium perfringens from chickens in Sichuan province. Chinese Journal of Zoonoses 25(8): 737-786.
Omidbakhsh N. 2010. Evaluation of sporicidal activities of selected environmental surface disinfectants: carrier tests with the spores of Clostridium difficile and its surrogates. American journal of infection control 38(9): 718-722.
Osland AM, Vestby LK, Nesse LL. 2020. The effect of disinfectants on quinolone resistant E. coli (QREC) in biofilm. Microorganisms 8(11): 1831.
Park JY, Kim S, Oh JY. Kim HR, Jang I, Lee HS, Kwon YK. 2015. Characterization of Clostridium perfringens isolates obtained from 2010 to 2012 from chickens with necrotic enteritis in Korea. Poultry science 94(6): 1158-1164.
Rood JI, Adams V, Lacey J, Lyras D, McClane BA, Melville SB, Moore RJ, Popoff MR, Sarker MR, Songer JG. 2018. Expansion of the Clostridium perfringens toxin-based typing scheme. Anaerobe (53): 5-10.
Shimizu T, Ohtani K, Hirakawa H, Ohshima K, Yamashita A, Shiba T, Ogasawara N, Hattori M, Kuhara S, Hayashi H. 2002. Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater. Proceedings of the National Academy of Sciences 99(2): 996-1001.
Tessari ENC, Cardoso ALSP, Kanashiro AMI, Stoppa GFZ, Luciano RL, de Castro AGM. 2014. Analysis of the presence of Clostridium perfringens in feed and raw material used in poultry production. Food Nutrition Sciences 2014.
Zhang C, Cui F, Zeng Gm, Jiang M, Yang Zz, Yu Zg, Zhu My, Shen Lq. 2015. Quaternary ammonium compounds (QACs): A review on occurrence, fate and toxicity in the environment. Science of the Total Environment (518): 352-362.
Zhang T, Zhang W, Ai D, Zhang R, Lu Q, Luo Q, Shao H. 2018. Prevalence and characterization of Clostridium perfringens in broiler chickens and retail chicken meat in central China. Anaerobe (54) 100-103.
 

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