Comparative biochemical and pathological studies between Toltrazuril and garlic supplementation in chickens infected with coccidiosis

doi:10.21608/ejah.2021.134362

Comparative biochemical and pathological studies between Toltrazuril and garlic supplementation in chickens infected with coccidiosis

Document Type : Original researches

10.21608/ejah.2021.134362

Abstract

Coccidiosis is one of the significant problems in the poultry industry throughout the world. It is responsible for 6–10% of all broiler mortality. The present study was conducted to evaluate the effect of combination between garlic powder and half therapeutic dose of toltrazuril for controlling clinical cecal coccidiosis, based on clinical signs, mortality rate ,oocysts counting, hematological, biochemical parameters of blood and pathological examination. One hundred at one day -old chicks were randomly divided into 5equal groups. The1^st group (A) that was kept uninfected (control negative group).The next (B, C, D and E) groups were infected with 20,000 sporulated oocysts per chick at the 20^th days- old. The group (B) that was infected but non treated (control positive).The group (C) that was treated with 7 mg/kg toltrazuril at 3^rddays P.I. which is the therapeutic dose. The group (D)was feed on ration containing garlic powder 8gm /kg ration as a prophylaxis. The group (E) was administered garlic powder 8gm/kg ration from the 1^st day age then given 3.5 mg/kg toltrazuril (half the therapeutic dose) at the 3 rd day P.I .In general the clinical signs were huddling ,severe depression , chilling ,reduction in the feed intake then profuse bloody dropping which decrease gradually by the day 11^th P.I. The mortalities were recorded on group (B,C and D).The hemato-biochemical parameters were significantly decreased in Hb, RBCs, PCV, serum protein level and significantly increased in TLC, AST ,ALT, Creatinine level .The pathological findings showed severe hemorrhagic enteritis, severe leucocytic infiltration and necrotic cecal glands. The birds in groups (E) showed mild symptoms with improvement of the clinical signs, averages feed intake, as well as hematological , biochemical parameters and pathological lesions with a decrease in oocyst number than in group (C).
It could be concluded that: the combination between garlic powder as prophylaxis and half dose of toltrazuril as treatment prevent the mortalities with reduction of the number of oocysts and improve hemato-biochemical parameters in the affected birds.

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Full Text

Comparative biochemical and pathological studies between Toltrazuril and garlic supplementation in chickens infected with coccidiosis

Shahira H.M. Hussein, Soad Mekawy, Mervat A. Ayoub , Gehan N.A. and Rania, A.A.

*Department of Pharmacology, **Department of Clinical Pathology, ***Department of Pathology and ****Department of Parasitology,

Animal Health Research Institute, Zagazig lab., ARHI, ARC

References

Adibmoradi M., Navidshad B., Seifdavati J.,and Royan M. (2006):- Effect of dietary garlic meal on histological structure of small intestine in broiler chickens. J Poultry Sci. 43:378–383.

Allen P.., DanfrthH.,and Augastien P.(1998):Dietary modulation of avian coccidiosis .Int.J. Parasitiol.; 28 1131-1140.

Bancroft J..and Stevens A. (1990) :Theory and practice of histological technique 3^rd E.D. Churchill Livingston,Edinburg, London Melbourne New Yourk.

Brown L.,Vrban E.and NewmankT..(1982):The birds of Africa.Vol.1 Academic Press Inc.London LTD ,UK.

Chapman H and Hacker A .(1994): Sensitivity of field isolates of Eimeria from two broiler complexes to anticoccidial drugs in chickens .Poult. Sci; 73(9)1404-1408.

David E , David G., and Richard .S.(1991) :Coccidiosis as acause of transmural lymphocytic enteritis and mortality in Captive Nashiville. Journal of wild life disease 27(4)615-620.

Desouky E, (2012)Prophilactic and therapeutic action of toltrazoril with coccivac B vaccination for prevention and control of coccidiosis in broiler chickens.Zag.vet.jornal Issn1110-1458.vol.no.3

Doumas,B.;Bayso ,D..;Carter R..;Peters,T. and Schaffer,R. (1980):Determination of serum total protein. Clin.Chem.,27: 1642.

El-Khatm ,AmiraAbdElLatif, and El-Hewaity.M (2014):- Efficacy of Turmeric (curcuma Longa)and garlic (allium sativum) on Eimeria species in broiler.International Journal of basic and applied Sciences 3(3) 349.

European MedicinesAgency; science medicines health (1998):-Committee for veterinary medicinal products toltrazuril; Summaryreport 1.EMEA/MRL/314/97 April.

Gedic N, Kabasakal L, Sehirli O, Ercan F, Sirvanci S , Keyeruysal M and Sener G (2005) : Long term administration of aqueous garlic extract (AGE) alleviates liver fibrosis and oxidative damage induced by biliary obstruction in rats .Life Sci 76:2593-2606

Gehan N.( 2005):-Pathological studies on some feed supplements in chickens infected with coccidiosis. A theseis of Ph. D. ; (pathology) presented by faculty of Vet . Med; Zagazig University.

Ghanem M..,RadwanM .,MoustafaA.andEbeidM.(2008):- Control of avian coccidiosis. Mode of Action: Toltrazurilinhibite nuclear division &microchondrial Comparative therapeutic effect of toltrazuril, sulphadimidine and amprolium on Eimeriabovis and Eimeriazuernii given at different times following infection in buffalo calves. (Bubalusbubalis).PreiveVet.Med.Animalmidecine Department.apr.17;84(1-2)161.

GiambroneJ. and Ronald P. (1986):- Vaccination of one day old broiler chicks against Newcastle disease and infectious disease during commercial life .Avian disease (30) 3: 557-562.

HalaM .(1995):- Pharmacological study on some anticoccidial drugs in chickens AtheseisM.V.Sc; (pharmacology) presented by faculty of Vet . Med; ZagazigUniversty.

Hassan. H.. El- Agmy S. Gaur R. , Fernando A. and Raj M.Ouhtit A(2009):- In vivo evidence of hepato-and reno-protective effect of garlic oil against sodium nitrite induced oxidative stress Int J BiolSci 5: 249-255

HiraniN . ,HasnaniJ .,Dhmi A.and Khanna K. (2007):- Hematobiological profile of broilers affected with coccidiosis..J Vet Parasit. 21(1):25-28.

Hodgson J . (1970) :Coccidiosisoocysts counting technique for coccidiostate evaluation . Exp. Parasitol; 28:99-102.

Jafari,R.Jalali M..andKiani R. (2012): Effect of fresh dietary garlic powder on some of serum biochemical parameters in broiler chicks. Comp ClinPathol 20 (4) : 295-297.

Jane E. and Mark G . (2014) :Antiprotozoal Drugs in canine and feline infectious diseases.Science Direct , 65(11):123-134.

Johnson J.and Reid W.(1970):- Anticoccidial drugs:lesion scoring techniques in battery and floor pen experiments with chickens. Exp. Parasitol. ;58(4);664-668.

Lakkundi J. ,Jagannath M. and Souza P.(2002): Effect of toltrazuril and amprolium on body weight and feed efficacy of broiler chickens experimentally infected with Eimeriatenella .Indian Jornals of Animal Sciences 72(10)935-937.

Long P ., Jpyner L. Millard B.and Norton C.(1976) :- A guide to laboratory technique used in study and diagnosis of avian coccidiosis. Folia Vetereniaria Latina,6:201-217.

Majed A.NailaC., Rifat U. and SinaGul (2019):Anticoccidial effect of garlic (Allium Sativium)and ginger( Zingiberofficinale)againistexperimentaly induced coccidiosis in broiler chickens.Journal of applied animal research Vol 47-1.

Mathis G. .Foryman,R and Kenndedy T.(2004):-Coccidiosis control by administratingtoltrazuril in the drinking water for 2 dayes period .Vet Parasito. 121-1-9.

Mona S .,(1998) :-Clinico-pathological studies on the aquazyme product in coccidial chickens . A thesis of M.V. Sc ; ( clinical pathology) presented by faculty of Vet . Med; Zagazig University.

Mondal, D..Chattopadhyay S., Batbayas S. Bera, A.., and Bhattacharya D. (2011): Plasma biochemical indices at various stage of infection with a field isolate of Eimeriatenella in broiler chicken. Vet World 4 (9) :404-409.

Noack S, Chapman HD, Selzer PM (2019): Anticoccidial drugs of the livestock industry. Parasitol Res 118:2009–2026.

Patra, G. Rajkhowa,T.. Ali,M.. Tiwary, J. .andSailo, L. (2010) : Studies on clinical, gross, histopathological and biochemical parameters in broiler birds suffered from Eimerianecatrix in Aizawl district of Mizoram,India. Int J Poult. Sci. 8 (11):1104-1106.

Peek,H,andLandmanW.(2011):Coccidiosis in poultry .Anticoccidialproducts,Vaccines and other prevention strategies.Vetrinary Quartery,31.143-161.

Pourali M.,GolianA,Razmi G. andSoukhtanlooM. (2014):Antioxidant and anticoccidial effect of garlic powder and sulpher amino acids on Eimeria- infected and uninfected broiler chickens.IranianJournal of Veterinary Research .4 (9). December

Rashid,M,AkbarH.,Alaraby,M.,Ismael A..Shehzaw. A. and Hashmi H.(2009):Comparative efficacy of different Tultrazuril doses against coccidiosis in broiler chickens.Mansoura,Vet.med. J.11(2):173-184.

Reitman, S. and Frankel, S. (1957):Acolorimetric method for determination of serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase.Am.J.Clin.Path., 25-56.

Sanders,G.andPasman, A. (1980): Determination of serum uric acid . Clin.Chem. Acta ., 101:299-303

SchalmO. ,Jain N., and Carrol E. (1975): Veterinary hematology . Lea and Febiger ,PhiladelphiaDoumas,B.T.Walson,W.A.and Biggs , H.G. (1971) : Albumin standerdsand the measurement of serum albumin with bromocresol green . Chem. Acta, 31-87

Soulsby E. (1982):Helminthes, Arthropods and protozoa of domestic animals .7^th Ed. BailliereTindall,London.. Clin.Chem.,27: 1642

Shawkat A.,ParulV.,Mohammad A. and IrfanA. (2014):Effect of garlic extract on haematobiochemical changes in Eimeriatenella infected broiler chickens .National academy of science ,37,311-316.

Shirely .M, (2000):The genome of Eimeria spp. with special reference to Eimeriatenella ;Acoccidium from chicken.Int.J. Parasitol.;30,485-493.

Solt, C. (1965):- Determination of serum cereatinine .J. Clin. Invest., 17,381.

Tamhane A. and Donlop D.(2000) : Statistics and data analysis from elementary to intermediate. Upper Saddle Rever, USA.

TewariK, Maharana B.(2011) ;Control of poultry coccidiosis .Journal ofparasitic disease 35:10-17

Wenlong Xiao, Xiaoyang Wang,Chunmei Wang, Mi Wang,Chenzhong Fei,Lifang Zhang,Feiqun Xue, Guoyong Wang and Keyu Zeyu Zhang (2019):Acute and 30-day oral toxicity studies of a novel coccidiostate- ethanamizuril.Toxico.Res.,8,686.

Youssef , D.. MansounT .Ramadan,A. andDessouki.B .(2007):Pathological study on the effect of some herbal plant as anticoccidial agents in broiler chickens .8^thScienceConf; Egyption Vet. Poltery Association